For quite a long time I am doing siRNA with different genes.
Lately I found out an interestin phenomena.
I am looking for the effect of kd of gene A on protein B. It seems that depending upon the amount of kd that I am getting the effect on protein B is changing. Reported to this effect is when you kd A B is increaing. I found that if the kd is ~40-50% I am getting an increase in B but if the kd is lower or higher 10-30% or 80-90% the effect is the opposite.
It is quite surprising.
Did any one got something similar?
Looking forward for a disscution in this topic.