siRNA transfection agent for mouse peritoneal macs

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sds
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siRNA transfection agent for mouse peritoneal macs

Can anyone suggest a good transfection agent for primary mouse peritoneal macs? I am planning to get the siRNA from Dharmacon. I've heard electroporation is good but would like to know more and it would be great if you could provide me a ref.

Thanks!

Jen_Floyd
Jen_Floyd's picture
I have had great success in

I have had great success in transfecting primary macrophages with DeliverX from Panomics. I optimized their standard protocol by doubling the DeliverX and halving the siRNA amount. Also for optimal stimulation later on (if that's what you're doing) seed 50-100k cells per 96 well.