Tet on cells

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Guy Sovak
Guy Sovak's picture
Tet on cells

Hi all,
I am looking for a tetracycline (TET ON) cell for transfection of siRNA gene.
Any one can help me?
Guy

Adolfo_Ferrando
Adolfo_Ferrando's picture
There are several tet

There are several tet regulated lines commercially available. However I would contact the group that published the RNAi tet inducible system and whatever experiment using their cells (if they are relevant for your question). I tried some of the commercilaly available lines and the level of regulation with psuperior was very poor.
Adolfo

Guy Sovak
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Dear Adolfo,

Dear Adolfo,
Could you please send me the authors of the paper.
I am also working with siRNA and Tet on, and some how I got the same effect with the 3t3 tet on cells with no siRNA inserted.
Thanks Guy

Adolfo_Ferrando wrote:

There are several tet regulated lines commercially available. However I would contact the group that published the RNAi tet inducible system and whatever experiment using their cells (if they are relevant for your question). I tried some of the commercilaly available lines and the level of regulation with psuperior was very poor.
Adolfo
vanishing
vanishing's picture
be careful about tetracyclin

be careful about tetracyclin in serum
(cattle being treated with tetracylin you will have basal expression)

you can obtain tet-free serum, but you cannot use that for immunofluorescence since it contains small charcoil particles

Guy Sovak
Guy Sovak's picture
Thanx,

Thanx,
I know it ,
Guy

vanishing wrote:

be careful about tetracyclin in serum
(cattle being treated with tetracylin you will have basal expression)

you can obtain tet-free serum, but you cannot use that for immunofluorescence since it contains small charcoil particles

yinilai
yinilai's picture
i ALSO WANT TO KNOW THE

i ALSO WANT TO KNOW THE PAPERS, COULD YOU TELL ME THAT?Adolfo_Ferrando wrote:

There are several tet regulated lines commercially available. However I would contact the group that published the RNAi tet inducible system and whatever experiment using their cells (if they are relevant for your question). I tried some of the commercilaly available lines and the level of regulation with psuperior was very poor.
Adolfo