What should be the total RNA sample loaded for siRNA blot analysis?

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gopal_joshii
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What should be the total RNA sample loaded for siRNA blot analysis?

Dear All,
Please suggest me how much total quantity required for nothern-blot analysis for siRNA library of plant sample.
Thanking you.
With regards.
Gopal
 

g a
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One of my friends is working

One of my friends is working on the animal tissue system .... they use as less as 50ng total RNA
However with plant system i dont know the optimal bu i guess more or less the basics remain same..... and they use autoradiographic detection systems

gopal_joshii
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Thank you very much Sir.  

Thank you very much Sir.