PhD Plant Biotechnology

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MUTHUMARIAPPAN ...
MUTHUMARIAPPAN Murugan's picture
PhD Plant Biotechnology

National Institute of Plant Genome Research(NIPGER), New Delhi (Department of Biotechnology) invites applications for the PhD Program.

Research Areas:

Frontier Plant Sciences such as
Developmental Biology,
Signal Transduction,
Stress Biology,
Structural,Functional and Nutritional Genomics,
Developing Transgenic Crop Plants etc.

Eligibility:
M. Sc. in Life Science or equivalent.
CSIR-UGC-NET / DBT-JRF /BET/BINC / ICMR-JRF

Application deadline: 09 June 2009

For more information visit..

http://www.nipgr.res.in/

Ehabzayed
Ehabzayed's picture
 

 
 
 
 

 
Name :                   EHAB MOHAMED ALI  ZAYED
Date of Birth:        22/06/1971.
Nationality :          EGYPTIAN .
Gander :                MALE
Education
2000 – 2007 : A Ph. D. candidate
Title : Improvment Of Soybean By Genetic Engineering Technologe .
summary of thesis will follow .
1994 – 1998 : master of science ( M. sc. )
Title : estimation of genetic variance in soybean
summary of thesis will follow .
1989 – 1993 : Under Graduate Student At The University Of Zagazig
Faculty Of Agriculture urned a B. Sc. Degree.
field of study for B. Sc. Was Agriculture Genetic And Genetic Engineering .
 
work of experience
 
1998 – present :
agriculturist ( researcher ) at the agriculture research center ( ARC ), field crops research institute (FCRI ) , cell research study department , forage corps research department and plant genetic rescources  department .
summary of my M. Sc.
 
This work was carried out at the experimental farw of the faclulty of agriculture and the laboratory of genetics department of faculty of agriculture , menofiya university , shebin – el-kom , Egypt during the growing seasons 1994 , 1995 and 1996 . five parents of soybean
Namely , harden (p1) , weber (p2) , forrest (p3) , calland (p4) , and clark (p5) and thrir lof , crosses produced from a half diallel crosses (zn – 1) --- were used as plant materials throughout this investigation . the results of this stady can be sumery all studied were performed on chemiced characterization .
(I)                Protein and oil contauts was determined
(II)             Study of fatty acid composition .
2. Enzyme Activities . studed the activates of three lipoxygenase isozymes , lipoxygenase isozymes ( lipoxygenase -i (L1) , lipoxygenase - ii ( l -2 ) and lipoxygenase iii (l – 3) of the same type of soybean mentioned .
ii – also , perocidase activity was determined in leaves before matwrity the genetic variance among f , crosses could be due mainly to non addative genetic variance .
iii – phenyloxidase activity
b- tissue culture reponse and dna content tissue culture response
* callus induction was initiated from immature supplemented with 3 mgL-1
ba 0.05 mg L-1naa .
results revealed that callus induction character is controlled by non addative .
** callus growth rate
callus was maintained on ms salts and b5 vitamines with 3 mgL-1BA- ASfor callus growth rate the addative and non addative hadmajor roles in the inheritance of this character .
*** DNA content and plant regeneration :
results of dna content revealed that heigh level of polyploidy in all genotyps under study ,therefore , regeneration into normal plantlet were unsuccessful with all genotypes .
c- earliness and growth attributes :
* earliness demonstrated heterosises
** growth attributes had the highly significant in the number of bromches per plant , number of pods per plant , first pod height and plant height yield and yiled components .
also , yield and yield components had the positive and highly sigrficant values for three parameters for heterotic effects for example 100- seed weight , number of seeds per pod , number of seeds per plant ….etc .
e- correlation coefficients
it apprared prescence of positive highly significant values for most studied characters and yield per plant .
mean while , there is anegative significant value for correlation between protein and oil contents , lipoxygenase –ii , iii and callus induction and yield per plant .
 
 
summary of my Ph.D.
    the experiments of the present study were conducted at field crops research institute (FCRI) , cell research study department , Agriculture Research Center (ARC) , Giza , Egypt during the growing seasons 2003 , 2004 and 2005 . fourteen soybean genotypes namely L89K-73,Corsay-79,Giza21,Forrest,Hutcheson,Calland,Lakota,Giza111,Giza83,Clak,Giza22,Giza35 ,Giza82 and Crowford were used as plant materials throughout this investigation,
The objectives of this study were:
(1)     Describe field perfomance of 14 soybean genotypes susceptible/moderate resistance/ resistante to cotton leaf worme and study the genetic paameters.
(2)     Initiatew and maintain callus organogensies cultures of soybean. The differences among soybean genotypes in callus formation were also evaluated.
(3)     Performed a system for soybean transformation and regeneration using immature embryos and cotledonary nodes
(4)     Use of DNA Markers in particular to their use in molecluar characterization for genetic improvmenet of cotton leaf worm of soybean.
The results of this study can be summerized as follow:
1-     Field Performance And Variation Of Soybean Genotypes.
2-     Tissue culture organogensises.
3-     Agrobacterium Etablishment Transformation System Of Soybean Using Immature Embryos And Cotledonary Nodes.
# in vito immatue embryo cotyledonary organogensises of  soybean(control).
# Kanamycine sensitivity.
# Time ofincoluation on Agrobacterium media.
# Kanamycine concentration.
# Production of tansfomed plantlet  by immature embryos.
# Production of tansfomed plantlet by Cotyledonary nodes.
4-     Molecluar characterization of soybean genotypes to resistant/Susceptible cotton leaf worm. Isozymes,Protein ,RAPD and RFLP
 

Training Courses

 

     
 
 
 
(1)   Egyptian Center For Industrial Safety And Training.
(2)   Science Witting(Enal).
(3)   Tissue Cluture(Center Laboratoy Of Date Palme).
(4)   Workshop About Biotechnology In Islamic Wold
(5)   Date Base Of Sciences (Enal).
(6)   Oil,Fiber And Forage Crops Breedingand Agronomy
(7)   Protein DNA And RNA Pactise And Wok On Bench Top (Faculty Of Postgraduate Alexandria University).
(8)   Winter Workshop 2006 And 2007 Fcri,Arc.
(9)   Banana,Tomato And Flower Plants Tissue Cluture(Shura Company)
(10)   Plant Transformation AGERI,ARC,Egypt.
(11)   ICDL
(12)   SPSS
(13)   Nanobiotechnology Workshop Program,Cairo University, Faculty of Science – 8 November 2008. Agriculture and Environment Session and Medical Session.
 
 
 
(14)   The secound conference of Field Cros research Institute"Shiping Future in Field crops" Organizing commit.11-13/10/2008
(15)   Work shop "Improvement of field Crops by non traditional Methods"10-16/8/2008 In FCRI,ARC.
(16)   Tools for Crops Improvments
(17)   Nontraditional methods to improve the field Crops(Lecturer and Coach)
(18)   Share with American university in Cairo biotechnology Conferrance

The number of publications

 

 
 
 

(1) Genetic performance of seed quality characters of five soybean(Glycine max(L.) Merril) varieties and their diallel crossess.Minufiya J. Of Agic.Vol.24(1999) No.3:999-1015.
(2) Field Performance and variation of soybean genotypes Susceptible/esistence to cotton leaf worm. Minufiya J. Of Agic.Vol.32(2007) No.1.
(3) SoybeanTissue Culture Organogensises.Mansoura J. Of Agic. (2006).
(4)Establishment Of Agrobacteium Tansformation System Using Immature Embryos And Cotyledonary Nodes In Soybean. First Field Crops Conference Proceeding 22-24,August 2006,88-100.
(5) Leaf anatomical evaluation of soybean genotypes susceptible/resistant to cotton leaf worm. First Field Crops Conference Proceeding 22-24,August 2006,498-509.
(6) Four publications will be published at this year about forage crops on:
·        Egyptian clover molecluar markers and Field Performance.
·        Egyptian clover Cytogenetics.
·        Egyptian Clover ISSR markers.
·        Zea mexicana anatomy and Indericte selection for quality Forage.
·        Zea mexicana comaprsion in field performance and DNA level.
·        Comaprsion between Fahal (Monocuts) and Helaly (Multycuts) in different molecluar markers (AFLP, RAPD,ISSR and Protein Profil)

Skills

 
·        Organizational committee of workshop

  • Organizational committee ofConference FCRI.
  • Setting workshop.
  • Computer and Internet.
  • Establishment of transformation system.
  • Tissue culture Lab from Design to Commercial Level.
  • DNA studies

Mailling

 

 
 
 

Home Adressess:
3th GALAB STREET FROM ZEDAIN,El- MATBAA,DAR EL-SALAM,CAIRO,EGYPT.
Telephone:-202025245563
Work Adressess:-
9 gamma street,ARC,FCRI, GIZA12619,EGYPT.
2020235731813.
E-MAIL:-
Ehabzayed31@hotmail.com
Ehabzayed2002@yahoo.com
Ehabzayed2007@gmail.com
 
 
 

 
Name :                   EHAB MOHAMED ALI  ZAYED
Date of Birth:        22/06/1971.
Nationality :          EGYPTIAN .
Gander :                MALE
Education
2000 – 2007 : A Ph. D. candidate
Title : Improvment Of Soybean By Genetic Engineering Technologe .
summary of thesis will follow .
1994 – 1998 : master of science ( M. sc. )
Title : estimation of genetic variance in soybean
summary of thesis will follow .
1989 – 1993 : Under Graduate Student At The University Of Zagazig
Faculty Of Agriculture urned a B. Sc. Degree.
field of study for B. Sc. Was Agriculture Genetic And Genetic Engineering .
 
work of experience
 
1998 – present :
agriculturist ( researcher ) at the agriculture research center ( ARC ), field crops research institute (FCRI ) , cell research study department , forage corps research department and plant genetic rescources  department .
summary of my M. Sc.
 
This work was carried out at the experimental farw of the faclulty of agriculture and the laboratory of genetics department of faculty of agriculture , menofiya university , shebin – el-kom , Egypt during the growing seasons 1994 , 1995 and 1996 . five parents of soybean
Namely , harden (p1) , weber (p2) , forrest (p3) , calland (p4) , and clark (p5) and thrir lof , crosses produced from a half diallel crosses (zn – 1) --- were used as plant materials throughout this investigation . the results of this stady can be sumery all studied were performed on chemiced characterization .
(I)                Protein and oil contauts was determined
(II)             Study of fatty acid composition .
2. Enzyme Activities . studed the activates of three lipoxygenase isozymes , lipoxygenase isozymes ( lipoxygenase -i (L1) , lipoxygenase - ii ( l -2 ) and lipoxygenase iii (l – 3) of the same type of soybean mentioned .
ii – also , perocidase activity was determined in leaves before matwrity the genetic variance among f , crosses could be due mainly to non addative genetic variance .
iii – phenyloxidase activity
b- tissue culture reponse and dna content tissue culture response
* callus induction was initiated from immature supplemented with 3 mgL-1
ba 0.05 mg L-1naa .
results revealed that callus induction character is controlled by non addative .
** callus growth rate
callus was maintained on ms salts and b5 vitamines with 3 mgL-1BA- ASfor callus growth rate the addative and non addative hadmajor roles in the inheritance of this character .
*** DNA content and plant regeneration :
results of dna content revealed that heigh level of polyploidy in all genotyps under study ,therefore , regeneration into normal plantlet were unsuccessful with all genotypes .
c- earliness and growth attributes :
* earliness demonstrated heterosises
** growth attributes had the highly significant in the number of bromches per plant , number of pods per plant , first pod height and plant height yield and yiled components .
also , yield and yield components had the positive and highly sigrficant values for three parameters for heterotic effects for example 100- seed weight , number of seeds per pod , number of seeds per plant ….etc .
e- correlation coefficients
it apprared prescence of positive highly significant values for most studied characters and yield per plant .
mean while , there is anegative significant value for correlation between protein and oil contents , lipoxygenase –ii , iii and callus induction and yield per plant .
 
 
summary of my Ph.D.
    the experiments of the present study were conducted at field crops research institute (FCRI) , cell research study department , Agriculture Research Center (ARC) , Giza , Egypt during the growing seasons 2003 , 2004 and 2005 . fourteen soybean genotypes namely L89K-73,Corsay-79,Giza21,Forrest,Hutcheson,Calland,Lakota,Giza111,Giza83,Clak,Giza22,Giza35 ,Giza82 and Crowford were used as plant materials throughout this investigation,
The objectives of this study were:
(1)     Describe field perfomance of 14 soybean genotypes susceptible/moderate resistance/ resistante to cotton leaf worme and study the genetic paameters.
(2)     Initiatew and maintain callus organogensies cultures of soybean. The differences among soybean genotypes in callus formation were also evaluated.
(3)     Performed a system for soybean transformation and regeneration using immature embryos and cotledonary nodes
(4)     Use of DNA Markers in particular to their use in molecluar characterization for genetic improvmenet of cotton leaf worm of soybean.
The results of this study can be summerized as follow:
1-     Field Performance And Variation Of Soybean Genotypes.
2-     Tissue culture organogensises.
3-     Agrobacterium Etablishment Transformation System Of Soybean Using Immature Embryos And Cotledonary Nodes.
# in vito immatue embryo cotyledonary organogensises of  soybean(control).
# Kanamycine sensitivity.
# Time ofincoluation on Agrobacterium media.
# Kanamycine concentration.
# Production of tansfomed plantlet  by immature embryos.
# Production of tansfomed plantlet by Cotyledonary nodes.
4-     Molecluar characterization of soybean genotypes to resistant/Susceptible cotton leaf worm. Isozymes,Protein ,RAPD and RFLP
 

Training Courses

 

     
 
 
 
(1)   Egyptian Center For Industrial Safety And Training.
(2)   Science Witting(Enal).
(3)   Tissue Cluture(Center Laboratoy Of Date Palme).
(4)   Workshop About Biotechnology In Islamic Wold
(5)   Date Base Of Sciences (Enal).
(6)   Oil,Fiber And Forage Crops Breedingand Agronomy
(7)   Protein DNA And RNA Pactise And Wok On Bench Top (Faculty Of Postgraduate Alexandria University).
(8)   Winter Workshop 2006 And 2007 Fcri,Arc.
(9)   Banana,Tomato And Flower Plants Tissue Cluture(Shura Company)
(10)   Plant Transformation AGERI,ARC,Egypt.
(11)   ICDL
(12)   SPSS
(13)   Nanobiotechnology Workshop Program,Cairo University, Faculty of Science – 8 November 2008. Agriculture and Environment Session and Medical Session.
 
 
 
(14)   The secound conference of Field Cros research Institute"Shiping Future in Field crops" Organizing commit.11-13/10/2008
(15)   Work shop "Improvement of field Crops by non traditional Methods"10-16/8/2008 In FCRI,ARC.
(16)   Tools for Crops Improvments
(17)   Nontraditional methods to improve the field Crops(Lecturer and Coach)
(18)   Share with American university in Cairo biotechnology Conferrance

The number of publications

 

 
 
 

 
(1) Genetic performance of seed quality characters of five soybean(Glycine max(L.) Merril) varieties and their diallel crossess.Minufiya J. Of Agic.Vol.24(1999) No.3:999-1015.
(2) Field Performance and variation of soybean genotypes Susceptible/esistence to cotton leaf worm. Minufiya J. Of Agic.Vol.32(2007) No.1.
(3) SoybeanTissue Culture Organogensises.Mansoura J. Of Agic. (2006).
(4)Establishment Of Agrobacteium Tansformation System Using Immature Embryos And Cotyledonary Nodes In Soybean. First Field Crops Conference Proceeding 22-24,August 2006,88-100.
(5) Leaf anatomical evaluation of soybean genotypes susceptible/resistant to cotton leaf worm. First Field Crops Conference Proceeding 22-24,August 2006,498-509.
(6) Four publications will be published at this year about forage crops on:
·        Egyptian clover molecluar markers and Field Performance.
·        Egyptian clover Cytogenetics.
·        Egyptian Clover ISSR markers.
·        Zea mexicana anatomy and Indericte selection for quality Forage.
·        Zea mexicana comaprsion in field performance and DNA level.
·        Comaprsion between Fahal (Monocuts) and Helaly (Multycuts) in different molecluar markers (AFLP, RAPD,ISSR and Protein Profil)

Skills

 
·        Organizational committee of workshop

  • Organizational committee ofConferenceFCRI.
  • Setting workshop.
  • Computer and Internet.
  • Establishment of transformation system.
  • Tissue culture Lab from Design to Commercial Level.
  • DNA studies

Mailling

 

 
 
 

 
Home Adressess:
3th GALAB STREET FROM ZEDAIN,El- MATBAA,DAR EL-SALAM,CAIRO,EGYPT.
Telephone:-202025245563
Work Adressess:-
9 gamma street,ARC,FCRI, GIZA12619,EGYPT.
2020235731813.
E-MAIL:-
Ehabzayed31@hotmail.com
Ehabzayed2002@yahoo.com
Ehabzayed2007@gmail.com
 

 
 
 

 
Name :                   EHAB MOHAMED ALI  ZAYED
Date of Birth:        22/06/1971.
Nationality :          EGYPTIAN .
Gander :                MALE
Education
2000 – 2007 : A Ph. D. candidate
Title : Improvment Of Soybean By Genetic Engineering Technologe .
summary of thesis will follow .
1994 – 1998 : master of science ( M. sc. )
Title : estimation of genetic variance in soybean
summary of thesis will follow .
1989 – 1993 : Under Graduate Student At The University Of Zagazig
Faculty Of Agriculture urned a B. Sc. Degree.
field of study for B. Sc. Was Agriculture Genetic And Genetic Engineering .
 
work of experience
 
1998 – present :
agriculturist ( researcher ) at the agriculture research center ( ARC ), field crops research institute (FCRI ) , cell research study department , forage corps research department and plant genetic rescources  department .
summary of my M. Sc.
 
This work was carried out at the experimental farw of the faclulty of agriculture and the laboratory of genetics department of faculty of agriculture , menofiya university , shebin – el-kom , Egypt during the growing seasons 1994 , 1995 and 1996 . five parents of soybean
Namely , harden (p1) , weber (p2) , forrest (p3) , calland (p4) , and clark (p5) and thrir lof , crosses produced from a half diallel crosses (zn – 1) --- were used as plant materials throughout this investigation . the results of this stady can be sumery all studied were performed on chemiced characterization .
(I)                Protein and oil contauts was determined
(II)             Study of fatty acid composition .
2. Enzyme Activities . studed the activates of three lipoxygenase isozymes , lipoxygenase isozymes ( lipoxygenase -i (L1) , lipoxygenase - ii ( l -2 ) and lipoxygenase iii (l – 3) of the same type of soybean mentioned .
ii – also , perocidase activity was determined in leaves before matwrity the genetic variance among f , crosses could be due mainly to non addative genetic variance .
iii – phenyloxidase activity
b- tissue culture reponse and dna content tissue culture response
* callus induction was initiated from immature supplemented with 3 mgL-1
ba 0.05 mg L-1naa .
results revealed that callus induction character is controlled by non addative .
** callus growth rate
callus was maintained on ms salts and b5 vitamines with 3 mgL-1BA- ASfor callus growth rate the addative and non addative hadmajor roles in the inheritance of this character .
*** DNA content and plant regeneration :
results of dna content revealed that heigh level of polyploidy in all genotyps under study ,therefore , regeneration into normal plantlet were unsuccessful with all genotypes .
c- earliness and growth attributes :
* earliness demonstrated heterosises
** growth attributes had the highly significant in the number of bromches per plant , number of pods per plant , first pod height and plant height yield and yiled components .
also , yield and yield components had the positive and highly sigrficant values for three parameters for heterotic effects for example 100- seed weight , number of seeds per pod , number of seeds per plant ….etc .
e- correlation coefficients
it apprared prescence of positive highly significant values for most studied characters and yield per plant .
mean while , there is anegative significant value for correlation between protein and oil contents , lipoxygenase –ii , iii and callus induction and yield per plant .
 
 
summary of my Ph.D.
    the experiments of the present study were conducted at field crops research institute (FCRI) , cell research study department , Agriculture Research Center (ARC) , Giza , Egypt during the growing seasons 2003 , 2004 and 2005 . fourteen soybean genotypes namely L89K-73,Corsay-79,Giza21,Forrest,Hutcheson,Calland,Lakota,Giza111,Giza83,Clak,Giza22,Giza35 ,Giza82 and Crowford were used as plant materials throughout this investigation,
The objectives of this study were:
(1)     Describe field perfomance of 14 soybean genotypes susceptible/moderate resistance/ resistante to cotton leaf worme and study the genetic paameters.
(2)     Initiatew and maintain callus organogensies cultures of soybean. The differences among soybean genotypes in callus formation were also evaluated.
(3)     Performed a system for soybean transformation and regeneration using immature embryos and cotledonary nodes
(4)     Use of DNA Markers in particular to their use in molecluar characterization for genetic improvmenet of cotton leaf worm of soybean.
The results of this study can be summerized as follow:
1-     Field Performance And Variation Of Soybean Genotypes.
2-     Tissue culture organogensises.
3-     Agrobacterium Etablishment Transformation System Of Soybean Using Immature Embryos And Cotledonary Nodes.
# in vito immatue embryo cotyledonary organogensises of  soybean(control).
# Kanamycine sensitivity.
# Time ofincoluation on Agrobacterium media.
# Kanamycine concentration.
# Production of tansfomed plantlet  by immature embryos.
# Production of tansfomed plantlet by Cotyledonary nodes.
4-     Molecluar characterization of soybean genotypes to resistant/Susceptible cotton leaf worm. Isozymes,Protein ,RAPD and RFLP
 

Training Courses

 

     
 
 
 
(1)   Egyptian Center For Industrial Safety And Training.
(2)   Science Witting(Enal).
(3)   Tissue Cluture(Center Laboratoy Of Date Palme).
(4)   Workshop About Biotechnology In Islamic Wold
(5)   Date Base Of Sciences (Enal).
(6)   Oil,Fiber And Forage Crops Breedingand Agronomy
(7)   Protein DNA And RNA Pactise And Wok On Bench Top (Faculty Of Postgraduate Alexandria University).
(8)   Winter Workshop 2006 And 2007 Fcri,Arc.
(9)   Banana,Tomato And Flower Plants Tissue Cluture(Shura Company)
(10)   Plant Transformation AGERI,ARC,Egypt.
(11)   ICDL
(12)   SPSS
(13)   Nanobiotechnology Workshop Program,Cairo University, Faculty of Science – 8 November 2008. Agriculture and Environment Session and Medical Session.
 
 
 
(14)   The secound conference of Field Cros research Institute"Shiping Future in Field crops" Organizing commit.11-13/10/2008
(15)   Work shop "Improvement of field Crops by non traditional Methods"10-16/8/2008 In FCRI,ARC.
(16)   Tools for Crops Improvments
(17)   Nontraditional methods to improve the field Crops(Lecturer and Coach)
(18)   Share with American university in Cairo biotechnology Conferrance

The number of publications

 

 
 
 

 
(1) Genetic performance of seed quality characters of five soybean(Glycine max(L.) Merril) varieties and their diallel crossess.Minufiya J. Of Agic.Vol.24(1999) No.3:999-1015.
(2) Field Performance and variation of soybean genotypes Susceptible/esistence to cotton leaf worm. Minufiya J. Of Agic.Vol.32(2007) No.1.
(3) SoybeanTissue Culture Organogensises.Mansoura J. Of Agic. (2006).
(4)Establishment Of Agrobacteium Tansformation System Using Immature Embryos And Cotyledonary Nodes In Soybean. First Field Crops Conference Proceeding 22-24,August 2006,88-100.
(5) Leaf anatomical evaluation of soybean genotypes susceptible/resistant to cotton leaf worm. First Field Crops Conference Proceeding 22-24,August 2006,498-509.
(6) Four publications will be published at this year about forage crops on:
·        Egyptian clover molecluar markers and Field Performance.
·        Egyptian clover Cytogenetics.
·        Egyptian Clover ISSR markers.
·        Zea mexicana anatomy and Indericte selection for quality Forage.
·        Zea mexicana comaprsion in field performance and DNA level.
·        Comaprsion between Fahal (Monocuts) and Helaly (Multycuts) in different molecluar markers (AFLP, RAPD,ISSR and Protein Profil)

Skills

 
·        Organizational committee of workshop

  • Organizational committee ofConferenceFCRI.
  • Setting workshop.
  • Computer and Internet.
  • Establishment of transformation system.
  • Tissue culture Lab from Design to Commercial Level.
  • DNA studies

Mailling

 

 
 
 

 
Home Adressess:
3th GALAB STREET FROM ZEDAIN,El- MATBAA,DAR EL-SALAM,CAIRO,EGYPT.
Telephone:-202025245563
Work Adressess:-
9 gamma street,ARC,FCRI, GIZA12619,EGYPT.
2020235731813.
E-MAIL:-
Ehabzayed31@hotmail.com
Ehabzayed2002@yahoo.com
Ehabzayed2007@gmail.com