PEI PEGylation

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esadeqzadeh
esadeqzadeh's picture
PEI PEGylation

I am working on non-viral gene delivery. I need to have a protocol on how to sheild my PEI/DNA nanoparticles with a bifunctional PEG. One head of the PEG is NHS desired to attach to the PEI and the other head is maleimide, desired to attach to my targeting antibody. I do not know how to manage this work, this will be needing the temporary blocking of the maleimide end when I want to attach it to the PEI and then removing the blockade to attach it to the targeting antibody.
Here is where I need help.
Will appreciate it very much