Primers for grass spp

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Dilip Fernando
Dilip Fernando's picture
Primers for grass spp

PLease let me know sequesnces for amplification of grass spp.
Is it Ok with rice primers to use amplify grass genes?
Any one with experience may reply me
Thank you

Herwig Just
Herwig Just's picture
hmmmm...

hmmmm...
the best thing you can do is do a blastn search with the primers and see if sequences are identical in the primer binding region

http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Nucleotides&PROGRAM=blastn&MEGABLAST=on&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch&SHOW_DEFAULTS=on

Dilip Fernando
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Thank you I'll try from there

Thank you
I'll try from there