gene-break transposons, zfishbook

1 post / 0 new
Jon Moulton
Jon Moulton's picture
gene-break transposons, zfishbook

This is gene engineering at the edge: "implementing gene-break transposons to create expression-tagged, revertible mutations in zebrafish genes".    http://zfishbook.org/ 

Here's a description of the technology:
Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC.  In vivo protein trapping produces a functional expression codex of the vertebrate proteome.  Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8.
http://www.nature.com/nmeth/journal/v8/n6/full/nmeth.1606.html

Clark KJ, Argue DP, Petzold AM, Ekker SC.  zfishbook: connecting you to a world of zebrafish revertible mutants.  Nucleic Acids Res. 2011;1–5. doi:10.1093/nar/gkr957
http://nar.oxfordjournals.org/content/early/2011/11/07/nar.gkr957.full