PCR problem

1 post / 0 new
arsonkamal
arsonkamal's picture
PCR problem

Hi
i m doing site-directed mutagenesis for DNMT1 gene. my desired size is 6kb, but after the pcr amplification i always get around 4kb,
i designed  a mutant primer (Tm=56.7,using pcr master mix as my DNA polymerase) and run pcr to mutant my gene, but i never get my desired size
i m seeking help