Question about generating probe from known AA sequence

1 post / 0 new
shie214
shie214's picture
Question about generating probe from known AA sequence

A mutation has been induced in the gene encoding a protein using the mutagen.  The resulting mutant protein can be purified and its amino acid sequence determined.   Using the amino acid sequences, one can determine the DNA sequence of this protein.  Question: how do you then generate a probe to identify the gene within a genomic library??

Question 2: suppose you found more than one gene, how would you distinguish a functioning gene from a pseudogene?