Restriction enzymes

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djneums's picture
Restriction enzymes


I am planning on buying the SAL1 restriction enzyme from new england biosystems. They don't offer DNA ligase in their kit. 

I'm trying to cut portions of two separate genes with SAL 1 and link them together. Given that SAL 1 produces 'sticky ends,', do I need ligase to link my two gene segments together after restriction digest? If so, do I need some specific type of ligase? 

If some one could direct me to a company that offers SAL1 with applications/reagent requirements, I would be very much appreciative,