shRNA silencing

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Lula
Lula's picture
shRNA silencing

Hello,

I am working with shRNA and I have to questions with respect to silencing with shRNA I would like to know.

Firstly, I've heard that shRNA linearization before transfection increases the probability of integration and reduces the time needed to select stable knockdown cell lines, is that true? Do you know any article related to this topic?

Secondly, for how long is shRNA inhibition stable? someone told me that silencing of the promoters after some weeks has been described but I can not find bibliography about this. Any idea?

Thanks a lot,

L.