Whole Genome Amplification

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KWaraska
KWaraska's picture
Whole Genome Amplification

Does anyone have any hard data or know of a publication that proves that WGA with phi29 does not affect the genotypes of the subsequent DNA? I have a customer who is reluctant to use WGA because they are afraid it will not genotype the same afterward.

rasap
rasap's picture
Hi,

Hi,
Fermentas states that phi29 provides unbiased amplification of human genome, see picture:
http://www.fermentas.com/catalog/modifyingenzymes/phi29dnapol.htm
and they also say that phi 29 is highly accurate, means makes not so many mistakes; for more you should refer to the article that they mentioned:
Esteban, J.A., et al., Fidelity of phi29 DNA polymerase, J. Biol. Chem., 268, 4, 2719-2726, 1993
Hopefully this helps a bit,
Regards