BigDye Xterminator problems anyone?

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CFergie
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BigDye Xterminator problems anyone?

We've been using ABI BigDye Xterminator to clean up our sequencing PCR rxns (spa & 16S) and are wondering if could be to blame for our high rate of sequencing failures.  sometimes as many as 1/3 of the 96 well plate fail.  We use the ABI 3130 instrument.  any other possibilities?  Thanks so much!

rajasereddy
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Hi

Hi
To answer this we need to know the entire process which u follow for cleanup. When did u start using Xterminator?

Biju
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CFergie

CFergie
I have not used the specific kit you have mentioned. But after doing over 1000 cycle sequeincg reacns with ABI reagents, my impression is that the failure could be more related to being careful at each step especially while transefrring from one tube to another.
Why dont try with a gene which used to work very well in teh lab and use the same batch of kit and see.
Biju Joseph