Detecting deletions via a sequencing trace?

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ucbccka
ucbccka's picture
Detecting deletions via a sequencing trace?

Can you do this? The sequence came through reporting a deletion, but when I look at the trace I see a double peak.

the sequence should read TTC TCA ACGT A

The trace says TTC TC- ACGT A

take a peek boys and girls. Any help would be appreciated.

cheers

Fraser Moss
Fraser Moss's picture
I read TTC TCA AGT A from the

I read TTC TCA AGT A from the image you posted.

I'd resubmit the sample and sequence on both strands to confirm.

Is this towards the end of the read? You might have some misreads going on if it is.

use a sequencing primer 100-200 bp away from the site for best results.