I want to isolate RNA and DNA from Anopheles mosquito samples. I have been trying different variations of the TRIzol protocol (back extraction buffer, sodium citrate) and have had little success in recovering good gDNA. My 260/230 ratios have generally been poor (0.30-0.60) and the absorbance peak at 270 nm! What's more puzzling is that when run on the gel, I get no bands!
I know there are some kits out there that can be a better alternative but I was wondering if anyone can recommend a particular kit from experience?