who has the protocol of mRNA 3' end library construction for solexa sequenc

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sxz
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who has the protocol of mRNA 3' end library construction for solexa sequenc

who has the protocol of mRNA 3' end library construction for solexa sequencing

ryan_m
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Could you elaborate on what

Could you elaborate on what you want to achieve? There is really no use in producing a 3' EST, so to speak with illumina/solexa, since you can generate sequences randomly derived from across entire mRNAs. Are you thinking of a SAGE-like approach? Or are you considering WTSS/RNA-seq?

Ryan