I had a isolated enterococcus strain. it has 2 plasmids of about 3 and 4 kb. i had pcr amplified the int gene long back. But when i started the work again...i am not getting any results..!
I checked the plasmid on gel and also quantified it...tried with different concentrations...but no amplification... and moreover i get the result with lysate ...but sometimes i dont.
Is it due to some contamination or is it that it might hve lost the gene....?