How to enrich glycopeptides well?

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Onee
Onee's picture
How to enrich glycopeptides well?

Dear all,

         I have digested some proteins from acylamide gel using trypsin and get the (glyco) peptides mixture. I want to analyze glycopeptides by MALDI-TOF-MS and also LC-MS/MS. Do you have any idea for enriching the glycopeptides? 

Thank a lot for your kindly help

Chin Fen Teo
Chin Fen Teo's picture
Hi Onee,

Hi Onee,

Do you have any idea what kind of glycan structures is present in your samples? If you do, you can always use lectin-agarose for en enrichment tool... And it works well with complex N-/O-glycopeptides.

Hope this helps, and good luck.

Onee
Onee's picture
Hi Pippuri

Hi Pippuri

         Many thank for your guide. My samples are highly N-glycosylated peptides. Now I plan to use Sepharose to enrith them but I not sure which one (between sepharose and any lectin) will give a better result. Anyway, what is the difference between sepharose CL-6B and 4B for glycan or glycopeptide enrichment?

Thank again.