Mitochondrial Dna isolation from human blood

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arun47biotech
arun47biotech's picture
Mitochondrial Dna isolation from human blood

hi
    if anyone knows the protocol of mitochondrial dna isolation from human blood pls helpme by telling the reagent preparataion, chemicla required with the protocol
                      thanking you

Arvind Singh Pundir
Arvind Singh Pundir's picture
Hi arun

Hi arun
here is a reference for your querry
Recovery of Mitoehondrial DNA from Blood Leukocytes Using Detergent Lysis
www.springerlink.com/content/w7642571724q0675/fulltext.pdf

bio.classes.ucsc.edu/bio187l/PDF%20files/Genomic%20DNA.pdf

ISOLATION OF GENOMIC AND MITOCHONDRIAL DNA

arun47biotech
arun47biotech's picture
hi thanks for your reply on

hi thanks for your reply on my queries, its really helpfull but still i have a doubt sir  in the protocol i.e  whether it isapplicable for human blood and then in the second one u have given the topic as ISOLATION OF GENOMIC AND MITOCHONDRIAL DNA but the methodology is for genomic dna whether it is applicable sir can i know ur email id and details for the further queries sir
                      thanking you sir

derin
derin's picture
No this is not the one..this

No this is not the one..this is genomic DNA not mitochondrial

Ranji
Ranji's picture
Hi Arun

Hi Arun
            Kindly mail me the protocol of mitochondrial dna isolation from human blood , the reagent preparataion, chemicals required for the protocol. Kindly reply at the earliest.
                  Thanking you

Biju
Biju's picture
Hi Ranji

Hi Ranji
Kits are avaialable for Qiagen for mitochndrial DNA isolation.

afshan.biotech
afshan.biotech's picture
HI PLS FORWARD ME PROYOCOL

HI PLS FORWARD ME PROYOCOL FOR MITOCHONDRIAL DNA ISOLATION. I NEED IT FOR MY PROJECT WORK

g a
g a's picture
 Hi Afshan

 Hi Afshan
you can download the protocol from the above mentioned link.
bio.classes.ucsc.edu/bio187l/PDF%20files/Genomic%20DNA.pdf

afshan.biotech
afshan.biotech's picture
Hi thanks 4 reply.

Hi thanks 4 reply.
i need a protocol to isolate mitochondrial DNA from blood. pls help........

g a
g a's picture
Hi Afshan

Hi Afshan

Check the following link
http://www.jstage.jst.go.jp/article/ggs/82/5/429/_pdf 
In case yr institutional library has the access, there is another good article 
BIOCHEMICAL GENETICS
Volume 30, Numbers 1-2, 27-33, DOI: 10.1007/BF00554425

afshan.biotech
afshan.biotech's picture
Thank you sir 4 ur reply. i

Thank you sir 4 ur reply. i already tried this but due to some problems i failed in isolating mtDNA. can u pls suggest me another protocol........ Thank you....