ASSAY FOR NATURAL KILLER CELL ACTIVITY

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Tony Rook
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ASSAY FOR NATURAL KILLER CELL ACTIVITY

Please find the link for the following protocol:

ASSAY FOR NATURAL KILLER CELL ACTIVITY

http://aactg.s-3.com/pub/download/imm/imnk.pdf

Principle:

NK cell activity is quantitated by determining the ability of peripheral blood mononuclear cells (PBMC) to lyse an NK sensitive target cell line (K-562). Target cells are labeled by incubation with radioactive chromium, washed, then added to a dilution series of PBMCs and incubated for 4 hours. Supernatants are harvested and the amount of intracellular chromium-51 released into the supernatant is measured with
a gamma counter. The amount of chromium-51 released is proportional to the lytic activity of the NK cells.