Help with CD90/Thy-1 immunostaining

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mtakkenberg
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Help with CD90/Thy-1 immunostaining

Has anyone ever done immunostaining of paraffin embedded sections with CD90/Thy-1 antibodies? I'm trying to get a working protocol, but I'm having a very hard time....
Can anyone help me out?

samm
samm's picture
You need to ensure you do the

You need to ensure you do the antigen retreival/exposure step (pressure cooking or microwaving) and queching before you stain. I have done this by pressure cooking in a citrate buffer.