LIMITING DILUTION ANALYSES TO DETERMINE HIV-SPECIFIC PCTL

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Tony Rook
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LIMITING DILUTION ANALYSES TO DETERMINE HIV-SPECIFIC PCTL

Please find the link for the following protocol:

LIMITING DILUTION ANALYSES TO DETERMINE HIV-SPECIFIC PCTL

http://aactg.s-3.com/pub/download/imm/imctl.pdf

Purpose and Description:

These methods measure precursor frequencies recognizing a specific HIV-1 gene product (method 1) or CTL epitope (method 2) by limiting dilution assay. The data are useful in determining quantitative changes in HIVspecific effector responses in a given individual over time. This should provide a better estimate of the effects of a given intervention on CTL responses than qualitative measurements of specific lysis to a given gene product.