Please find the link for the following protocol:
LIMITING DILUTION ANALYSES TO DETERMINE HIV-SPECIFIC PCTL
Purpose and Description:
These methods measure precursor frequencies recognizing a specific HIV-1 gene product (method 1) or CTL epitope (method 2) by limiting dilution assay. The data are useful in determining quantitative changes in HIVspecific effector responses in a given individual over time. This should provide a better estimate of the effects of a given intervention on CTL responses than qualitative measurements of specific lysis to a given gene product.