Master Plex vs. Graph Pad for ELISA curve fitting

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bpani
bpani's picture
Master Plex vs. Graph Pad for ELISA curve fitting

Hello guys,
I'm been working with ELISAs and Master Plex for quite a while and never had a problem.
Recently I changed labs and the new place use Graph Pad for curve fitting.
When we run the same ELISA assay in both softwares the results were differents.
Someone now why this may be happening? THe softwares work differently for 4PL curves?
Any ideas?

Cheers