Morpholinos to validate antibodies

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Jon Moulton
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Morpholinos to validate antibodies

Here's an example of using Morpholinos to validate target specificity of antibodies.
Belgacem YH, Borodinsky LN. Inversion of Sonic hedgehog action on its canonical pathway by electrical activity. Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):4140-5. doi: 10.1073/pnas.1419690112. Epub 2015 Mar 17.
"To prove specificity of anti-Gli2 and anti-Gli3 antibodies used in this study, X. laevis Gli2- and Gli3-targeted translation-blocking morpholinos (Gli2-MO: GCACAGAACGCAGGTAATGCTCCAT; Gli3-MO: GACTGGGCTTCCATGTTGTCTTCTC) were unilaterally (Gli2-MO) or bilaterally (Gli3-MO) injected at the two-cell-stage. Embryos were processed for Western blot or immunohistochemistry assays and probed with indicated antibodies."