I'm learning how to isolate PBMCs but do not know how much whole blood to start with if I need to have a final count of 10 million cells in each cryovial for storage.
I need about 200 vials to store as my control.
Does anyone know how many PBMCs are in 1ml of whole blood??
How many mL of whole blood do I need to isolate 2 billion PBMCs (10 million PBMCs in 200 cryovials).