Peritoneal Macrophage Cultures - how long do they keep?

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Stef26
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Peritoneal Macrophage Cultures - how long do they keep?

Hi,

I'm about to try to isolate these cells for the first time and manipulate them. Are these cell cultures generally tricky to work with?

I specifically want to know how long these cultures keep for. My plan was to take the peritoneal macrophages from the same mice that I take another cell type from in order to do eventual co-culture experiments. Will pMacrophages stay in culture for 2 weeks? If so, what medium do i put them in? Anyone know if they respond to growth factors (EGF/FGF2)?

Thanks