I'm about to try to isolate these cells for the first time and manipulate them. Are these cell cultures generally tricky to work with?
I specifically want to know how long these cultures keep for. My plan was to take the peritoneal macrophages from the same mice that I take another cell type from in order to do eventual co-culture experiments. Will pMacrophages stay in culture for 2 weeks? If so, what medium do i put them in? Anyone know if they respond to growth factors (EGF/FGF2)?