ELISA

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Hasan
Hasan's picture
ELISA

Salaam for all,

I am trying to investigate the effect of flavonoids on IL-6 and TNF alpha secretion in Caco-2 cells and HRT-18.

should I first stimulate Caco-2 before treatment with flavonoids or without stimulation, and if I should stimulate tumor cell lines, what is the best stimulant is it conA, LPS or IL-1B to release IL-6 and TNF alpha by Caco-2 cells and what is the best concentration for these stimulant and the duration of exposure.

Hasan.

University of Jordan.