Looking for a naive human phage scFv library

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reza tohidkia
reza tohidkia's picture
Looking for a naive human phage scFv library

I am intrested in immunotherapy of gastric cancer with scFv. I am going to be screening a phage scFv library against a tumor associated antigen of gastric cancer. However i don't know how to earn a naive human phage scFv library. Also does anyone know which non-immune human phage scFv library is appropriate for my study.

hilltrekker
hilltrekker's picture
Hi

Hi
Please go through this link for a 22-page booklet about naive human phage scFv library.
http://www.eigenkapitalforum.com/062/Workshopspdf/Biotech%20-%20Private%20Company%20Forum_Affimed%20therapeutics%20AG_Fischer.pdf
Also find pasted  below an abstract from pubmed
1: Methods Mol Biol. 2009;525:129-42, xv.
Construction of a large naïve human phage-displayed Fab library through one-step cloning.
Zhu Z, Dimitrov DS.
NCI-Frederick, National Institutes of Health, Frederick, MD, USA.
Antibody-based therapeutics is attracting more attention in the post-genome era, in contrast to a diminution in the initial high expectation for rapid development of gene-based therapeutic modalities. In support to the antibody-based therapeutics, the advent of recent technologies has made human antibody screening and production progressively more economic. Among those technologies, phage-display antibody library has been successfully applied in the antibody-based drug development both as fully human antibody sources and tools for antibody engineering. Building up a high-quality antibody library with a large library size and high diversity has been crucial for successful isolation of antibodies. Here we describe an efficient strategy for the construction of a large naïve phage-display human Fab library with one-step cloning. Optimization of each key step is extensively discussed and simplified protocols for library panning and Fab production are also described.
PMID: 19252833 [PubMed - indexed for MEDLINE
I hope it helps. 

reza tohidkia
reza tohidkia's picture
Hi

Hi

Thanks alot. Do you think that i can buy this phage antibody library or the other one such as the Human Combinatorial Antibody Library (HuCA)
The other word, i am giong to buy one for my investigation

computer
computer's picture
I don't think that you can

I don't think that you can buy a library. First of all they will not give you, if they, than it will cost like anything. you can give your antigen to them and they may screen their library against that and will send you two or three possilble ScFv s. But you can try buying it but please tell me the cost too.

reza tohidkia
reza tohidkia's picture
Hi.

Hi.
Are you interested in Phage antibody library too?

reza tohidkia
reza tohidkia's picture
Hi

Hi
Do anyone know how to obtain a phage scFv antibody library?

computer
computer's picture
Yes, if we can get it.

Yes, if we can get it.

reza tohidkia
reza tohidkia's picture
Hi. What do you mean?

Hi. What do you mean? Eventually, may I get a phage antibody library from you?