Streptavidin Magnetic Beads

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drseidl
drseidl's picture
Streptavidin Magnetic Beads

I will be using magnetic beads for immunoprecipitation.  I've been referred to a company called Solulink from a colleague of mine for the actual beads.  I'm new to this process and need to know if the NanoLink Magnetic Beads will work for me.  If so, should I use the streptavidin, 4FB or amino version?  The size is 0.8 nm.  Any ideas?  Any help someone could give me on using beads for immunoprecipitation would really help.

Also, what other applications can I use these beads for?  Sorry if these questions sound too elementary...

Dynabeads
Dynabeads's picture
Hi,

Hi,

A very nice alternative for immunoprecipitation experiments is Dynabeads. 

General information
Dynabeads are non-porous uniform, superparamagnetic, monodispersed highly cross-linked polystyrene microsphere consisting of an even dispersion of magnetic material throughout the bead. 
The magnetic beads are coated with a thin of highly cross-linkedpolystyrene shell which encases the magnetic material and prevent any leakage from the beads or trapping of ligands in the bead interior. Also it provides a defined surface area for the adsorption or coupling of various molecules. Uniformity of bead size and shape provides consistent physical and chemical properties.

The immunomagnetic principle:  

The principle of immunomagnetic separation is very simple. First, a tube of functionalized Dynabeads are mixed with the sample containing the target. Due to a very short binding kinetics most of the target will bind during the first 10-15 minutes. Due to the iron content of the beads in combination with a very strong magent the target can be pulled out of the solution within seconds. After removing the supernatant the isolated target can alternatively be eluted off the beads for downstream applications.

Dynabeads and IP
Main advantages of using Dynabeads in IP is time due to rapid binding kinetics, low background, high reproducibility and easy handling. No pre-clearing is required.

Dynabeads and Non-covalent binding of Abs
Non-covalent coupling binding of Ab to Dynabeads can be done by using  Dynabeads Protein G (cat no 100.03D) since Protein G has affinity towards the Fc part of the Abs. Both stand-alone beads and a kit where all IP buffers are included (cat no 100.07D) are available. Alternatively, Dynabeads pre-coupled with secondary Abs can be used.

Dynabeads and covalent binding of Abs
Covalent binding of Abs to Dynabeads can be done by using one of the surface activated beads, most easily done by using the Antibody coupling kit (Cat no 14311D) where all reagents required are included.

Dynabeads and elution of target
compatible with:
- elution by using low pH
- elution with ionic strength
- denaturing conditions

Kind regards
Ketil

Ketil Winther Pedersen (Ph.D)
Knowledge Leader
Dynal Bead Based Separations
Invitrogen Group, Invitrogen Dynal AS
Tel: +47 22 06 11 10 (direct)
Norway
ketil.pedersen@lifetech.com