Enhance neutralizing epitope

3 posts / 0 new
Last post
dylee
dylee's picture
Enhance neutralizing epitope

hello
      did anyone do any reseach on enhancing the immunity of neutralizing epitope,since they are just a few AAs,can not induce strong response .i want to add a few bps to it ,but i do not know how and where i can get some knowledge of these things.

xm833
xm833's picture
There are a variety of

There are a variety of approaches you can try to enhance immunogenicity. Some of the most straightforward that I have used are fusions to 'universal' T cell epitopes (a quick pubmed search will give you plenty of hits). These are small sequences derived from highly immunogenic proteins which can induce immunogenicity of sequences to which they are linked. There is no guarantee that any one T cell epitope will automatically induce immunogenicity, so I typically would make a few different ones and see which worked.

You may also consider fusions with the C3d epitope which binds receptors on B cells and enhances antigen presentation or looking into adjuvants (co injection of cytokines such as GM-CSF)

dylee
dylee's picture
Thanx for your suggestions

Thanx for your suggestions.Would you please like to send me your paper about what you have done about universal t cell epitopes? eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%77%6f%61%69%6e%69%5f%38%36%30%32%30%32%40%31%36%33%2e%63%6f%6d%22%3e%77%6f%61%69%6e%69%5f%38%36%30%32%30%32%40%31%36%33%2e%63%6f%6d%3c%2f%61%3e%27%29%3b'))
Thank you very much