I will use cytokines for the first time. I have 10µg of a cytokine and I will suspend it in 1ml of PBS, so I will have a concentration of 10µg/ml.
I need different concentrations of this cytokine: 10ng/ml, 100ng/ml, 1ng/ml and 0.1ng/ml…
I thought that I should start by preparing a solution of 10µg/ml then from this solution I will take 1µl that I suspend in 999µl of PBS to get a concentration of 10ng/ml.
For a concentration of 100ng/ml I will take 10µl from the solution of 10µg/ml that I add to 990µl of PBS.
Please is that correct? If it’s not how should I proceed?
Thank you very much
Cytokine concentrations helpppppp