intracellular cytokine detection

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kafi
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intracellular cytokine detection

 Hello! I would like to detect human intracellular IFNb by Western Blot. Does anybody know a protocol or suitable antibody. Also, do you know if it is feasable to detect IFNb from cell lysates by ELISA? Thanks for any suggestions.

heehawmcduff
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Hi Kafi

Hi Kafi

A colleague of mine used this Abcam antibody a while back.  I don't have a protocol but it apparently worked well.  I'll see if I can get the dilutions he used.

http://www.abcam.com/Interferon-beta-antibody-PBS-03-ab6979.html

It is certainly possible to detect IFNb in lysate.

Robertwe
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