intracellular cytokine detection

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kafi's picture
intracellular cytokine detection

 Hello! I would like to detect human intracellular IFNb by Western Blot. Does anybody know a protocol or suitable antibody. Also, do you know if it is feasable to detect IFNb from cell lysates by ELISA? Thanks for any suggestions.

heehawmcduff's picture
Hi Kafi

Hi Kafi

A colleague of mine used this Abcam antibody a while back.  I don't have a protocol but it apparently worked well.  I'll see if I can get the dilutions he used.

It is certainly possible to detect IFNb in lysate.

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View this presentation in real time and free on October 18, 2012 - Towards an in vitro assay for vaccine immunogenicity

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