I am trying to double stain for Mast cells and ST2 receptor to show the percent of Mast cells that have the ST2 receptor.
The mast cell antibody works great by itself. I usually see about 15% mast cells. The ST2 receptor also works great by itself, with about 70% cells with ST2 receptor.
HOWEVER, when I double stain them, the Mast cell antibody does not show up more than 2%. It seems that the ST2 antibody is interfering with the mast cell antibody?
The ST2 antibody is made by Santa Cruz, (C-20). It is goat polyclonal IGG
The mast cell antibody is BD pharmingen mouse anti rat clone ar32aa4
Why would these not work together ???