I am looking for a kit to Isolate Mouse T regulatory cells for further In Vivo assay. The existing kits are relying on the presence of CD4 and CD25 on the cell which could be common marker for both T reg and effector T cells. I am wondering if there is any suggestion to isolate Pure T regulatory from mouse spleen. Should I rely on the absence of CD127 (does it work in mice also)? or any other suggestion.
Isolation of MOUSE T regulatory T cells