T cell proliferation using CFSE

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cst3376
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T cell proliferation using CFSE

Hi all... Actually, I have some problem related to T cell proliferation using CFSE. The results which I got were iDC showed to have higher ability in proliferating T cells than LPS (10ng/mL)-DCs in allogeneic system. I keep optimize but I couldn't solve it. Basically, I coculture the DC:T cells with different ratio, which are 1:10, 1:5 and 1:3 and the T cell proliferation showed a decrease pattern with the increase of cell ratio when compare with iDCs and LPS-DCs.
Do anyone know what actually happened?