The major goals of the research program are to delineate how the cell division cycle is regulated in unperturbed cycling cells (cell cycle control); how cell division is delayed by replicative- and genotoxic-stress (checkpoint control); how cancer cells derail these regulatory pathways and ultimately; to use this information to treat human disease. Clinical, preclinical and basic studies in breast cancer are a major focus of the laboratory. We are actively involved in designing and analyzing the results of Phase I/II clinical trials aimed at translating our fundamental knowledge of cell cycle- and checkpoint-control into improved targeted therapies for breast cancer patients. Recognizing that a key challenge facing breast cancer researchers today is the lack of good preclinical models for studying human breast cancer, we are working with primary human breast tumors obtained directly from breast cancer patients. These tumors are being propagated in the humanized mammary fat pads of immune compromised mice for our preclinical studies (HIM model). Many of these models metastasize out of the mouse mammary gland to distant mouse organs, including bone and lung. We are identifying the molecular changes associated with the acquisition of metastasis in this model. Much of our mechanistic understanding of mammalian cell cycle control has come from studying cells cultured in vitro. It is unclear whether the regulatory pathways discovered using cultured cells are operational in the context of a living, breathing vertebrate animal. Actively developing mouse models that enable regulatory pathways to be studied non-invasively and repetitively in living mice.
1. Responsible for the day-to-day operation of the laboratory.
2. Responsible for lab compliance, including but not limited to Environmental Health and Safety and Radiation Safety compliance and HIPPA compliance.
3. Responsible for keeping laboratory up-to-date on protocols, including but limited to the use of vertebrate animals and the use of human specimens.
4. Responsible for assessing new technologies and laboratory equipment.
5. Responsible for upkeep and overseeing repair of laboratory equipment.
6.Responsible for mentoring, training, and providing oversight/advice to educational appointments and classified research staff regarding experiments involving all aspects of sterile technique and molecular biology techniques (nucleic acid isolation, cloning, quantitation, Southern, Northern and Western blotting and analysis).
7.Assist with writing, editing and publishing papers and grant applications on a consistent basis.
8.Capable of performing experiments that involve handling of mice as well as cells, tissues and tumors derived from mice.
9.Perform experiments with human-derived cells and tissues.
10. Experience with or willingness to learn how to overproduce proteins in cells by transfection, electroporation or viral infection (lentiviruses, retroviruses, adenoviruses and baculoviruses).
11.Other duties as assigned.
Required: Bachelor's degree in one of the natural sciences or related field.
Preferred: Master's degree in one of the natural sciences or related field.
Required: Seven years experience in scientific, research or clinical laboratory, to include four years in a supervisory/managerial capacity. With preferred degree, five years of required experience to include four years in a supervisory/managerial capacity.
Deadline: 18 April 2014