Use of electronic-pipette

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Shubhangi
Shubhangi's picture
Use of electronic-pipette

Hi
I am sure many of us must be using e-pipettes. How comfortable are you using it? For me it works best for dispensing but problem is for performing serial dilutions. It is because the pipettes takeup some back volume after dispensing.
Does anyone come across this? How you are correcting this?
 

RAVISHPATEL
RAVISHPATEL's picture
Dear Mam

Dear Mam
 
I have been passed from same problem initially when I perform E-pet.
In such case while you are performing serial dilutions first blow pipette out-side the solution and press enter button & than immediately deep pipettes tips in solution which you want to do serially dilute because during blow condition pipette suck out some air & which generate bobble at initial level.
So do this practice only in first well.
But once it finish in first well you will have no problem for rest well.
And the query for back volume after dispensing, it will take in all E-pet which will be on pip-max mode but you need not to worry about this as this is preset back volume which will not considered in final volume.
 
(Back volume will be same from first well to last well & at the end we have to throw the solution so according to this we have to first calibrate our system)
 
Ravish
 

heehawmcduff
heehawmcduff's picture
I have been using electronic

I have been using electronic multichannel pipettes in the lab for a few years now and I have to say that they are fantastic.  I only make one small change when I am using them, and that is, depending on the volume, to hold the pipette tips just above the surface of the liquid that I am dispensing into.  This is because, on the final blow-out, the pipette dispenses extra air and that inevitably causes bubbles. 
But otherwise, they are great!

Ivan Delgado
Ivan Delgado's picture
The trick I use is to listen

The trick I use is to listen to the beeps the e-pipette gives you (I am not sure if all models have this feature). You will get one beep when it dispenses and then a second beep when it takes up the back volume (about a second or two later). All you do is place the tips on the wells, let it dispense, and then you hear the first beep you list the tips away from the dispensed solution. The pipette will take up air instead of your solution. This works great for most solutions. If your solution is viscous though, I suggest you do what Ravishpatel suggested.

andy123
andy123's picture
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p { margin-bottom: 0.08in; }
Hi, I would suggest you that electronic pipette the Eppendorf Xplorer is specially designed for users working with complex or long pipetting series. It stands for exact setting of parameters, maximum reproducibility of results and low operating force. The intuitive operating concept and ergonomic display set new standards in simplicity, precision and reproducibility.

way2jordan
way2jordan's picture
I also face these type of

I also face these type of problem because when we use this we have to follow some points 1. Pre wet the pipette tip 2. Work at temperature equilibrium 3. Examine the tip before dispensing the sample. 4. Pull the pipette straight out. 5. Use standard mode pipetting. 5. Use constantly after aspiration. 7. Minimize handling of pipette and tip. 8. Immerse the tip to the proper depth. 9. Use the correct pipette tip. 10. Use consistent plunger pressure and speed.
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pipette tips to use e-pepette

handsomejohanna
handsomejohanna's picture
 Hi, I would suggest you that

 Hi, I would suggest you that electronic pipette the Eppendorf Xplorer is specially designed for users working with complex or long pipetting series. The pipette will take up air instead of your solution. This works great for most solutions.