I am new to the world of Reverse Transcription PCR and would really appreciate some advice about whether it is the right technique for me or not…
I have the full genome sequence of the bacteria I am working with, I had previously designed and DIG labelled probes specific for the operon I am studying (for northern blotting experiments). BUT…
Is it possible to use RT-PCR to follow expression?
Would the probes need to be redesigned?
(& if so what is the best technique for designing them?)
What company is best to purchase the reagents from?
Sorry if any part of my query is unclear, please do not hesitate to contact me for any further information.