Confined evolution on petri dishes - Looking for a Protocol

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Nucleum
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Confined evolution on petri dishes - Looking for a Protocol

I'm looking for a protocol to perform confined evolution on yeasts... I'm trying to stimulate the yeast to grow better on a specific carbon source by natural mutations.

can anyone help me?? I need some kind of protocol for this process occur in petri disshes

varsha
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wrote:

wrote:

The following strategy is based on isolation of natural mutants for food sources or drugs. We tried something like this with P. falciparum, which is not yeast.
you make plates with your source of Carbon and streak your yeast on it. It would take some time, but eventually you would see colonies coming up.
If they are real mutants, they would grow faster if you grow them on similar plates.
Specific protocols are available in papers.