Gfp

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alyssafuent
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Gfp

Can GFP be used for staining bacteria? How can gfp be extracted from a tissue of an animal?

The FFM
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 alyssafuent

 alyssafuent
Can you be more specifc in your description of what it is that you are wanting to see with the GFP?  Are you getting the bacteria to express soluble GFP in the cytoplasm or are you fusing a protein expressed in the bacteria with the GFP in order to vizulaize it or sort the expressing cells by FACS?

And yes if GFP is expressed at good enough levels you can extract GFP from tissue using IP with many of the commercially available monoclonal and polyclonal anti-GFP antibodies.