Measuring reactive oxygen species

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Phillippe
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Measuring reactive oxygen species

Hello! I seed my SH-SY5Y cells onto a 96 well plate with 2*10^4 cells/well and then I incubate my cells with 10 µM DCFH-DA for 30 min before treatment. I use h202 as a positive control and it works. Nevertheless the variation in my samples is HUGE. I suspect it has to do with the washing steps where some wells lose a lot of cells while others lose less. Is there any way i can normalize for cell numbers on a 96 well plate