Hi, I'm beggining a project involving measuring expression levels in early Drosophila embryos. However, I need to be sure that the freshly laid eggs I collect are fertilized. I'm going to be using species other than Drosophila melanogaster, so I cannot rely on transgenics to make sure that they're fertilized.
Does anyone know of a method to test for fertilization that involves chemical washing or somethign similar? I've heard that, because of their chorion coat, fertilized eggs are more resistant to certain chemicals (e.g., bleach) than unfertilized eggs, however, simply being activated, which happens before laying, will cause eggs to be quite resistant to bleach. Any advice would be helpful.