Superdex 200

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pauline teoh
pauline teoh's picture
Superdex 200

I am doing about the nanorods conjugate antibody research work. I tried to purify my end product by using self-packed superdex 200 colum. I found that the nanorods solution stuck at top of the gel, and no have antibody eluted out. I cofirmed the fraction by using SDS-PAGE method.
 Any suggestion about that. I would apreaciate the suggestion.
 Thanks.

Bettye
Bettye's picture
 Are your nanorods soluble in

 Are your nanorods soluble in water? You could try diafiltration:

Sweeney, S.F., G.H. Woehrle, and J.E. Hutchison “Rapid Purification and Size Separation of Gold Nanoparticles via Diafiltration,” J. Am. Chem. Soc. 2006128, 3190-319