I am trying to record espontaneous activity in orgnotypic cultured slices in cell attached voltage clamp. From looking at Perkins, K.L. (2006) J Neurosci Methods 154 (1-2): 1-18, the most appropriate method is loose seal cell attached voltage clamp. However, I cannot find any information relating to what magnitude of seal should be used - i.e. what is the optimal seal to use when recording in the loose seal configuration? Does anyone have an opinion as to what seal resistance is the best one to use?