Electrophysiology

2 posts / 0 new
Last post
Tyro
Tyro's picture
Electrophysiology

 I have been working on this since months! I have a problem with increasing currents (+ve/ -ve). I get good seal and whole cell. But the current increases/decreases slowly with each sweep. I tried everything n checked everything, including the osmolarities of the solutions. I am not able to figure out what's going wrong. It is really discouraging. can any one please help me to troubleshoot the situation.

JamesDL
JamesDL's picture
Does the current also drift

Does the current also drift with the model cell on the head stage?

If not, and assuming you are using silver wire in your patch pipette, have you tried chloridating/rechloridating it?

James