patching dentate gyrus

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jack_prem
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patching dentate gyrus

Hello,
I have been patching Dentate gyrus pyramidal cells and do current clamp recording for the past couple of days. I use Multiclamp 700B, pClamp 10.2 and K-Gluconate as internal solution. Whenever I patch dentate gyrus pyramidal cells, I can get 1GOhm seal but as soon as I switch from voltage clamp to current clamp, the cells are depoalrised to -30 mV. Does anyone know how to fix it?

Thanks

James