Apply lipid solution into PC12 cells

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renxnana's picture
Apply lipid solution into PC12 cells

 I am working with the anti-inflammatory property of emu oil. I have a problem in applying the lipid into the pc12 cells. Is there any method i can use to dissolve the lipid to induce the pc12 cells? thanks.

The FFM's picture
 Look up preparation of

 Look up preparation of Micelles 

Radoslav Savic, Tony Azzam, Adi Eisenberg, Hinyu Nedev, Lawrence Rosenberg, Dusica Maysinger,

Block-copolymer micelles as carriers of cell signaling modulators for the inhibition of JNK in human islets of Langerhans

Biomaterials, Volume 30, Issue 21, July 2009, Pages 3597-3604, ISSN 0142-9612, DOI: 10.1016/j.biomaterials.2009.03.028.


Here we investigate the potential of PCL-b-PEO micelles in preventing the cell death of isolated human islets of Langerhans. PCL-b-PEO micelles were loaded with c-Jun NH2-terminal kinases inhibitor SP600125 to rescue the isolated islets. Mechanistic studies of the uptake were conducted in PC12 cells. Incorporation of SP600125 afforded 8.2 fold greater solubility of SP600125 in micelle suspension. To investigate the effectiveness of micelle-incorporated SP600125 in preventing the islet cell death, we challenged the islets with TNF-alpha, IL-1, and IFN gamma. Micelle-incorporated SP600125 did not lose its inhibitory activity during incorporation into micelles, and it protected the islets against cytokine-induced loss of viability to the same extent as control SP600125. Moreover, the concentration of micelle-incorporated SP600125 used was 13-fold lower, demonstrating the greater efficacy of micelle delivered SP600125. Micelles maintained their cytoplasmic distribution without detectable nuclear localization in islets. The inhibition of JNK was confirmed by western blots. This study suggests that micelle-based intracellular delivery of potent, poorly water soluble, cell-death-pathway inhibitors may represent a valuable addition to established delivery of cytocidal block-copolymer micelle-incorporated bioactives.

Keywords: Micelle; Islet; Drug delivery; Diabetes; Polycaprolactone; Polyethyleneoxide