Phosphoric Acid, How to make it

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nessa88
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Phosphoric Acid, How to make it

Hey to everyone,

  I just started working at a new job and i have an assignment due in two days regarding the testing of valerian root extract. I am getting low results and I am trying different mobile phases. I need to know how to make a mobile phase of methanol:0.5% phosphoric acid (80:20). I was thinking of making into two separate mobile phases if possible. can someone tell me if that is possible or if not, how do i make the mobile phase of methanol:0.5% phosphoric acid (80:20). thanks.

g a
g a's picture
Hi

Hi

Commercially available phosphoric acid is usually 88% Phosphoric acid.

TO prepare 0.5% from this you need to take 568microliter of this pure acid and make the volume 100ml.

Now take 80ml of methanol and add the solution above to final volume of 100ml.

* Do not add methanol to phosphoric acid but add phosphoric acid to methanol.