Hey to everyone,
I just started working at a new job and i have an assignment due in two days regarding the testing of valerian root extract. I am getting low results and I am trying different mobile phases. I need to know how to make a mobile phase of methanol:0.5% phosphoric acid (80:20). I was thinking of making into two separate mobile phases if possible. can someone tell me if that is possible or if not, how do i make the mobile phase of methanol:0.5% phosphoric acid (80:20). thanks.