Has anyone worked with transplatin in cell culture tox assays and found it to be toxic ?
I'm working with the drug (300 micromolar) using short incubation times (2 hours) in ovarian cancer cells. I'm getting reproducible IC50 values 24 h later of around 150 micromolar.
The drug is dissolved in EBSS by vortexing and brief sonication using a probe sonicator on low setting.
Many thanks for any thoughts